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US 2020/0400679 A1Published application
Ligand discovery for t cell receptors
AssigneesThe Board of Trustees of the Leland Stanford Junior University, California Institute of Technology
InventorsMichael Edward Birnbaum, Juan Luis Mendoza, Michael Thomas Bethune, David Baltimore, Kenan Christopher Garcia
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Abstract
Compositions and methods are provided for the identification of peptide sequences that are ligands for a T cell receptor (TCR) of interest, in a given MHC context.
Claims21
-27. (canceled)
A method of identifying a set of peptide ligands that bind to a T cell receptor (TCR) of interest in a specific MHC protein context, the method comprising:
- contacting the TCR of interest with a population of host cells comprising a cell surface library of single chain polypeptides each comprising (a) a peptide ligand and (b) a binding domain of an MHC protein;
- selecting host cells expressing a single chain polypeptide that binds to the TCR of interest;
- iterating the selecting step for at least three rounds to obtain a selected population of host cells;
- and
- performing deep sequencing of the selected population of host cells to provide a dataset of sequences of the set of peptide ligands.
2 more elements
Dependencies are inferred from the claim text.