Methods for cancer detection by evaluation of glycan-binding patterns of immunoglobulins in gastrointestinal lavage fluid samples

A method for detecting a glycan-binding pattern associated with pancreatic cancer or predisposition thereto in a subject, comprising:

• obtaining a sample comprising gastrointestinal lavage fluid (GLF) from the subject, wherein the GLF comprises immunoglobulin A (IgA);
• contacting the GLF with a plurality of glycans;
• and
• detecting binding between the IgA and the plurality of glycans, thereby detecting the glycan-binding pattern.

A method for evaluating or monitoring the progression of pancreatic cancer or the efficacy of a pancreatic cancer treatment in a subject via a glycan-binding pattern associated with pancreatic cancer or predisposition thereto, comprising:

• obtaining a sample comprising gastrointestinal lavage fluid (GLF) from the subject, wherein the GLF comprises immunoglobulin A (IgA);
• contacting the GLF with a plurality of glycans;
• and
• detecting binding between the IgA and the plurality of glycans, thereby detecting the glycan-binding pattern.

A method for diagnosing a gastrointestinal (GI) tract cancer or predisposition thereto in a subject, comprising:

• obtaining a sample comprising gastrointestinal lavage fluid (GLF) from the subject, wherein the GLF comprises immunoglobulin A (IgA);
• contacting the GLF with a plurality of glycans;
• and
• detecting binding between the IgA and the plurality of glycans, thereby detecting a glycan-binding pattern;
• wherein the glycan-binding pattern is associated with the GI tract cancer or predisposition thereto.

A method for detecting a glycan-binding pattern associated with a gastrointestinal (GI) tract cancer or predisposition thereto in a subject, or evaluating or monitoring the progression of GI tract cancer or the efficacy of GI cancer treatment comprising:

• obtaining a sample comprising gastrointestinal lavage fluid (GLF) from the subject, wherein the GLF comprises immunoglobulin A (IgA);
• contacting the GLF with a plurality of glycans;
• and
• detecting binding between the IgA and the plurality of glycans, thereby detecting the glycan-binding pattern.