Paper-based collection and test devices for biological samples
AssigneeOhio State Innovation Foundation
InventorsAbraham K. BADU-TAWIAH, Deidre E. DAMON, Suji LEE, Benji FREY
Life of this patent
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Abstract
Disclosed herein systems, apparatuses and methods for the collection and testing of biological samples, and more specifically to rapid and simple methods for diagnosis of various diseases, conditions, or symptoms via the testing of collected biological samples on paper devices.
Claims27
A method of detecting at least one antigen in a biological sample, comprising:
- a) contacting the biological sample with a hydrophobic cellulose substrate comprising a capture antibody;
- b) contacting the substrate with a reporter antibody to form a capture antibody-antigen-reporter antibody sandwich complex;
- c) washing the substrate to remove unbound reporter antibody;
- d) reacting the reporter antibody to generate a reporter compound;
- and
- e) detecting the reporter compound using mass spectrometry.
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11-13. (canceled)
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An assay device comprising a plurality of porous layers, comprising:
- a) a reagent layer comprising a reporter antibody disposed in a reporter region;
- b) a capture layer comprising a capture antibody disposed in a capture region, wherein said capture antibody is conjugated to the porous substrate of the capture layer;
- c) a detection layer comprising a hydrophobic cellulose substrate;
- wherein the reporter region is in fluid communication with the capture region, and the detection layer is in fluid communication with the capture region.
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A substrate for paper spray mass spectrometry, comprising a triangular shaped hydrophobic cellulose substrate, the substrate includes a base and first and second edges, the first and second edges intersect the base at first ends thereof, and a sum of the angles at which the first and second edges intersect the base is greater than 135 degrees, wherein the substrate comprises silane-functionalized cellulose, and wherein after the substrate is contacted with biological sample and dried, the resulting dried sample is in the shape of a spheroid, wherein the distance from the surface of the substrate to the highest point in the spheroid is at least 50% the diameter of the spheroid.
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Dependencies are inferred from the claim text.