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US 2020/0400657 A1Published application
Antibody screening methods
AssigneeX-Body, Inc.
InventorsYan CHEN, Tod M. WOOLF, Richard W. WAGNER
Life of this patent
Estimating the term…
Abstract
Provided are methods and compositions for the production of novel antibodies that bind specifically to a target antigen. These methods and compositions are particularly useful for producing antibodies having the antigen binding specificity of a reference antibody but with improved properties (e.g., binding affinity, immunogenicity, and thermodynamic stability) relative to the reference antibody.
Claims12
1-14. (canceled)
A method for selecting a bispecific, stable VH/VL pair, the method comprising:
- (a) providing an unpaired VH domain that binds specifically to a first antigen;
- (b) contacting the unpaired VH domain with a library of unpaired VL domains such that a library of VH/VL pairs is formed;
- (c) contacting the library of VH/VL pairs with a second antigen;
- (d) selecting from the library of VH/VL pairs at least one VH/VL pair that binds specifically to the second antigen;
- (e) contacting the VH/VL pair(s) selected in step (d) with the first antigen;
- and
- (f) selecting at least one VH/VL pair that binds specifically to the first antigen, thereby selecting a bispecific, stable VH/VL pair.
4 more elements
An oligonucleotide having a sequence selected from the group consisting of SEQ ID NOs:
- 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 70, 71, 72, 73, 74, 75, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, and 102.
Dependencies are inferred from the claim text.