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US 2020/0400652 A1Published application
Ex vivo subcutaneous injection model
AssigneeGenoskin
InventorsPascal DESCARGUES, Emeline PAGÈS, Claire JARDET
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AssigneeGenoskin
InventorsPascal DESCARGUES, Emeline PAGÈS, Claire JARDET
Estimating the term…
Disclosed is an ex vivo model for subcutaneous injection and aims at providing an in vitro method including the steps of i) immersing a skin explant in a solidifiable liquid matrix such that the upper face of the epidermis is not covered, which matrix is itself contained in a cell culture insert, the bottom of which consists of a porous membrane, and (ii) solidifying this matrix so as to trap the immersed portion of this skin explant, wherein the upper face of the epidermis is not covered, and adhering this same matrix to the side walls and the porous membrane of the insert, wherein the skin explant includes a thickness of at least 5 mm of hypodermis.
An in vitro method comprising the steps of:
A cell culture insert being an ex vivo model for subcutaneous injection, which can be placed in a container or culture well, which has a bottom consisting of a porous membrane, and which contains the skin explant trapped in a solidified matrix which is in contact with the inner edge of the insert and the porous membrane, and wherein the epidermis of the skin explant is in contact with the atmosphere, and the dermis, epidermal appendages, and hypodermis of this skin explant are immersed in the solidified matrix.
-9. (canceled)
A kit comprising a an cell culture insert being an ex vivo model for subcutaneous injection, which can be placed in a container or culture well, which has a bottom consisting of a porous membrane, and which contains the skin explant trapped in a solidified matrix which is in contact with the inner edge of the insert and the porous membrane, and wherein the epidermis of the skin explant is in contact with the atmosphere, and the dermis, epidermal appendages, and hypodermis of this skin explant are immersed in the solidified matrix and an automatic injection device.
Dependencies are inferred from the claim text.