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US 2020/0400537 A1Published application
Compositions and Methods for Biological Sample Processing
AssigneeGen-Probe Incorporated
InventorPatrick Lynn Peterson
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AssigneeGen-Probe Incorporated
InventorPatrick Lynn Peterson
Estimating the term…
Methods, apparatuses, and systems are provided for processing a biological sample. Exemplary methods comprise transferring a swab associated with an aliquot of the biological sample into a wash liquid, wherein a first portion of the aliquot is released into the wash liquid; and transferring the swab into a carrier liquid, wherein a second portion of the aliquot is released into the carrier liquid. The methods can provide efficient transfer of analyte such as cells or nucleic acid into the carrier liquid while releasing particulate matter, viscous polymers, or other undesired material into the wash liquid. Alternatively or in addition, the methods can also release desired material into the wash liquid, e.g., cells, which can be used in applications such as culturing.
A method of processing a biological sample, the method comprising:
The method of any one of the preceding claims, wherein the biological sample comprises particulate matter.
The method of any one of the preceding claims, wherein the biological sample comprises at least one viscous polymer.
The method of any one of the preceding claims, wherein the biological sample comprises a lipid.
The method of any one of the preceding claims, wherein the biological sample comprises a lipid phase or a lipid emulsion.
The method of any one of the preceding claims, wherein the biological sample comprises stool.
The method of any one of the preceding claims, wherein the biological sample comprises sputum, mucus, blood, soil, sludge, or a microbial growth.
The method of any one of the preceding claims, wherein the biological sample is of plant or animal origin.
The method of any one of the preceding claims, wherein the biological sample is of mammalian origin.
The method of any one of the preceding claims, wherein the biological sample is of human origin.
The method of any one of the preceding claims, wherein the biological sample is obtained from a food.
The method of any one of the preceding claims, wherein the swab undergoes agitation in the wash liquid to release the first portion of the aliquot into the wash liquid.
The method of any one of the preceding claims, wherein the swab undergoes agitation in the carrier liquid to release the second portion of the aliquot into the carrier liquid.
The method of any one of the preceding claims, wherein the swab comprises a tip and the tip comprises flocked fibers.
The method of any one of the preceding claims, wherein the swab comprises a tip and the tip comprises wrapped fibers.
The method of any one of the preceding claims, wherein the swab becomes associated with a volume of 1 to 500 microliters of the biological sample in step (a).
The method of any one of the preceding claims, wherein the biological sample comprises or is suspected of comprising at least one cell.
The method of any one of the preceding claims, wherein the biological sample comprises or is suspected of comprising at least one bacterium.
The method of any one of the preceding claims, wherein the biological sample comprises or is suspected of comprising at least one spore.
The method of any one of the preceding claims, wherein the biological sample comprises or is suspected of comprising at least one virus.
The method of any one of the preceding claims, wherein the biological sample comprises or is suspected of comprising at least one pathogen.
The method of any one of the preceding claims, wherein the wash liquid is aqueous.
The method of any one of the preceding claims, wherein the wash liquid comprises a salt.
The method of any one of the preceding claims, wherein the wash liquid comprises a detergent.
The method of any one of the preceding claims, wherein the wash liquid has an osmolarity of 0 to 1000 mOsm.
The method of any one of the preceding claims, wherein the wash liquid is present within a vessel and the wash liquid has a volume of 0.2 to 2 ml.
The method of any one of the preceding claims, wherein the carrier liquid is aqueous.
The method of any one of the preceding claims, wherein the carrier liquid comprises a protease.
The method of any one of the preceding claims, wherein the carrier liquid comprises an RNase inhibitor.
The method of any one of the preceding claims, wherein the carrier liquid comprises a divalent cation chelator.
The method of any one of the preceding claims, wherein the carrier liquid is a lysis solution.
The method of any one of the preceding claims, wherein the carrier liquid or lysis solution comprises a detergent.
The method of any one of the preceding claims, wherein the carrier liquid is present within a vessel and the carrier liquid has a volume of 0.2 to 10 ml.
The method of any one of the preceding claims, wherein the biological sample comprises cells and the method further comprises lysing cells after step (c).
The method of any one of the preceding claims, wherein the biological sample comprises viruses having a coat or envelope and the method further comprises releasing nucleic acid from the coat or envelope.
The method of any one of the preceding claims, wherein the biological sample comprises at least one target nucleic acid, and the method further comprises capturing the target nucleic acid with at least one capture probe or isolating the target nucleic acid by precipitation or chromatography.
The method of any one of the preceding claims, further comprising determining the presence or absence of at least one macromolecule in the second portion of the biological sample.
The method of any one of the preceding claims, further comprising quantifying at least one macromolecule in the second portion of the biological sample.
The method of any one of the preceding claims, further comprising amplifying at least one nucleic acid from the second portion of the aliquot.
The method of any one of the preceding claims, further comprising sequencing at least one nucleic acid from the second portion of the aliquot or an amplicon thereof.
The method of any one of the preceding claims, further comprising hybridizing a detection probe to at least one nucleic acid from the second portion of the biological sample or an amplicon thereof.
The method of any one of the preceding claims, further comprising performing an invasive cleavage assay to detect at least one nucleic acid from the second portion of the biological sample or an amplicon thereof.
The method of any one of the preceding claims, wherein the biological sample is a crude sample.
The method of any one of the preceding claims, further comprising contacting the biological sample with the swab, wherein the aliquot of the biological sample and the swab become associated.
The method of any one of the preceding claims, wherein the first portion of the aliquot released into the wash liquid comprises cells.
Dependencies are inferred from the claim text.