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US 2020/0399710 A1Published application
Method of identifying tumor specific macromolecular isoforms
AssigneeIsommune, LLC
InventorChristian Barrett
Life of this patent
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Abstract
The present disclosure provides methods for the identification of tumor-specific isoforms of polymorphic gene expression products. Methods of using said isoforms in the diagnosis and/or treatment of cancers and neoplasias are also provided.
Claims21
A method of making a synthetic polynucleotide, comprising:
- selecting a cellular compartment;
- identifying a first set of genetic sequences expressed in said cellular compartment;
- identifying a second set of genetic sequences expressed as multiple isoforms;
- generating a subset of said first and second sets of genetic sequences, said subset comprising sequences that are both (a) expressed in said cellular compartment and (b) capable of being expressed in multiple isoforms;
- and
- generating polynucleotide primers that are complementary to one or more sequences from said subset of genetic sequences, and may further comprise sequences providing for the use of universal sequencing primers and/or unique genetic identifiers.
3 more elements
A method of obtaining a tumor-specific biomarker, comprising:
- selecting a cellular compartment;
- identifying a first set of genetic sequences expressed in said cellular compartment;
- identifying a second set of genetic sequences expressed as multiple isoforms;
- and
- generating a subset of said first and second sets of genetic sequences, said subset comprising one or more genetic sequences known to be both (a) expressed in said cellular compartment and (b) capable of being expressed in multiple isoforms;
- and
- extracting, purifying, or expressing the product of one or more genetic sequences from said subset of genetic sequences.
4 more elements
A method of generating antibodies against a tumor-specific biomarker, comprising:
- selecting a cellular compartment;
- identifying a first set of genetic sequences expressed in said cellular compartment;
- identifying a second set of genetic sequences expressed as multiple isoforms;
- and
- generating a subset of said first and second sets of genetic sequences, said subset comprising sequences that are both (a) expressed in said cellular compartment and (b) capable of being expressed in multiple isoforms;
- and
- extracting, purifying, or expressing the product of one or more genetic sequences from said subset of genetic sequences;
- and
- generating an antibody against said product.
6 more elements
21-27. (canceled)
Dependencies are inferred from the claim text.