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AssigneeFundaçãO Oswaldo Cruz
InventorsIVANO RAFAELLE VICTORIO DE FILIPPIS CAPASSO, ANTONIO EUGENIO CASTRO CARDOSO DE ALMEIDA
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The present invention provides a real-time PCR method that allows in a single step the simultaneous detection of etiological agents of bacterial meningitis, more specifically, Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae. For this, primers were used to amplify particular regions of the genomes of said bacteria. The presence of bacteria in a sample is indicated by the presence of amplicon, which is detected by means of detection methods appropriate to the PCR methodology employed.
Oligonucleotide, characterized by the fact that it is able to bind to a region of the Neisseria meningitidis nspA gene, and is suitable as a primer, said oligonucleotide comprising at least 10 to 15 consecutive nucleotides of the selected sequences of SEQ ID NOs:
Oligonucleotide, characterized by the fact that it is able to bind to a region of the Haemophilus influenzae P6 gene, and is suitable as a primer, said oligonucleotide comprising at least 10 to 15 consecutive nucleotides from the selected sequences of SEQ ID Nos:
Oligonucleotide, characterized by the fact that it is able to bind to a region of the Streptococcus pneumoniae ply gene, and is suitable as a primer, said oligonucleotide comprising at least 10 to 15 consecutive nucleotides from the selected sequences of SEQ ID Nos:
Oligonucleotide, characterized by the fact that it is able to bind to the nspA gene segment of Neisseria meningitidis, and is suitable as a probe, said oligonucleotide probe comprising at least 8 to 15 consecutive nucleotides of the selected sequence of SEQ ID NO:
Oligonucleotide, characterized by the fact that it is able to bind to the P6 gene segment of Haemophilus influenzae, and is suitable as a probe, said oligonucleotide probe comprising at least 8 to 15 consecutive nucleotides of the selected sequence of SEQ ID NO:
Oligonucleotide, characterized by the fact that it is able to bind to the ply gene segment of the Streptococcus pneumoniae, and is suitable as a probe, said probe oligonucleotide comprising at least 8 to 15 consecutive nucleotides of the selected sequence of SEQ ID NO:
Oligonucleotide set, characterized by the fact that it comprises at least two oligonucleotides selected from sequences comprising SEQ ID NOs:
Dependencies are inferred from the claim text.