An immunochromatographic analysis device for detecting chikungunya virus, comprising a sample loading part, a labeling substance retaining part, a chromatographic medium part having a detection part, and an absorption part, wherein
- the labeling substance retaining part contains the following antibody (A) or an antigen-binding fragment thereof, and
- the detection part contains the following antibody (B) or an antigen-binding fragment thereof, and the following antibody (C) or an antigen-binding fragment thereof,
- (A) an antibody against the chikungunya virus, including the following heavy chain variable region (1) and the following light chain variable region (2),
- (1) a heavy chain variable region including a heavy chain complementarity determining region (CDRH) 1, CDRH2, and CDRH3,
- CDRH1 being a polypeptide containing the following amino acid sequence (H1-A),
- CDRH2 being a polypeptide containing the following amino acid sequence (H2-A), and
- CDRH3 being a polypeptide containing the following amino acid sequence (H3-A),
- (H1-A) the following amino acid sequence (H1-A1), (H1-A2), or (H1-A3),
- (H1-A1) an amino acid sequence of SEQ ID NO:
- 1,
- (H1-A2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO:
- 1,
- (H1-A3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO:
- 1,
- (H2-A) the following amino acid sequence (H2-A1), (H2-A2), or (H2-A3),
- (H2-A1) an amino acid sequence of SEQ ID NO:
- 2,
- (H2-A2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO:
- 2,
- (H2-A3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO:
- 2,
- (H3-A) the following amino acid sequence (H3-A1), (H3-A2), or (H3-A3),
- (H3-A1) an amino acid sequence of SEQ ID NO:
- 3,
- (H3-A2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO:
- 3,
- (H3-A3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO:
- 3,
- (2) a light chain variable region including a light chain complementarity determining region (CDRL) 1, CDRL2, and CDRL3,
- CDRL1 being a polypeptide containing the following amino acid sequence (L1-A),
- CDRL2 being a polypeptide containing the following amino acid sequence (L2-A), and
- CDRL3 being a polypeptide containing the following amino acid sequence (L3-A),
- (L1-A) the following amino acid sequence (L1-A1), (L1-A2), or (L1-A3),
- (L1-A1) an amino acid sequence of SEQ ID NO:
- 4,
- (L1-A2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO:
- 4,
- (L1-A3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO:
- 4,
- (L2-A) the following amino acid sequence (L2-A1), (L2-A2), or (L2-A3),
- (L2-A1) an amino acid sequence of SEQ ID NO:
- 5,
- (L2-A2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO:
- 5,
- (L2-A3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO:
- 5,
- (L3-A) the following amino acid sequence (L3-A1), (L3-A2), or (L3-A3),
- (L3-A1) an amino acid sequence of SEQ ID NO:
- 6,
- (L3-A2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO:
- 6,
- (L3-A3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO:
- 6,
- (B) an antibody against the chikungunya virus, including the following heavy chain variable region (3) and the light chain variable region (2),
- (3) a heavy chain variable region including a heavy chain complementarity determining region (CDRH) 1, CDRH2, and CDRH3,
- CDRH1 being a polypeptide containing the following amino acid sequence (H1-B),
- CDRH2 being a polypeptide containing the following amino acid sequence (H2-B), and
- CDRH3 being a polypeptide containing the following amino acid sequence (H3-B),
- (H1-B) the following amino acid sequence (H1-B1), (H1-B2), or (H1-B3),
- (H1-B1) an amino acid sequence of SEQ ID NO:
- 7,
- (H1-B2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO:
- 7,
- (H1-B3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO:
- 7,
- (H2-B) the following amino acid sequence (H2-B1), (H2-B2), or (H2-B3),
- (H2-B1) an amino acid sequence of SEQ ID NO:
- 8,
- (H2-B2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO:
- 8,
- (H2-B3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO:
- 8,
- (H3-B) the following amino acid sequence (H3-B1), (H3-B2), or (H3-B3),
- (H3-B1) an amino acid sequence of SEQ ID NO:
- 9,
- (H3-B2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO:
- 9,
- (H3-B3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO:
- 9,
- (C) an antibody against the chikungunya virus, including the following heavy chain variable region (4) and the light chain variable region (2),
- (4) a heavy chain variable region including CDRH1, CDRH2, and CDRH3,
- CDRH1 being a polypeptide containing the following amino acid sequence (H1-C),
- CDRH2 being a polypeptide containing the following amino acid sequence (H2-C), and
- CDRH3 being a polypeptide containing the following amino acid sequence (H3-C),
- (H1-C) the following amino acid sequence (H1-C1), (H1-C2), or (H1-C3),
- (H1-C1) an amino acid sequence of SEQ ID NO:
- 10,
- (H1-C2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO:
- 10,
- (H1-C3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO:
- 10,
- (H2-C) the following amino acid sequence (H2-C1), (H2-C2), or (H2-C3),
- (H2-C1) an amino acid sequence of SEQ ID NO:
- 11,
- (H2-C2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO:
- 11,
- (H2-C3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO:
- 11,
- (H3-C) the following amino acid sequence (H3-C1), (H3-C2), or (H3-C3),
- (H3-C1) an amino acid sequence of SEQ ID NO:
- 12,
- (H3-C2) an amino acid sequence having 80% or more identity with the amino acid sequence of SEQ ID NO:
- 12,
- (H3-C3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted, and/or added in the amino acid sequence of SEQ ID NO:
- 12.