Recombinant production method
AssigneeHoffmann-La Roche Inc.
InventorsTobias KILLIAN, Markus NEUBAUER
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Abstract
Herein is reported a method for the recombinant production of a polypeptide in a eukaryotic cell comprising the steps of (i) cultivating a eukaryotic cell comprising a nucleic acid encoding the polypeptide in a cultivation medium comprising a compound selected from the group consisting of trans-2-methyl 2-pentenoic acid, the broad-spectrum HDAC inhibitor Quisinostat, and the subtype-specific HDAC inhibitor Romidepsin, and (ii) recovering the polypeptide from the cell or the cultivation medium and thereby producing the polypeptide in a eukaryotic cell.
Claims23
A method for the recombinant production of a polypeptide in a eukaryotic cell comprising the steps of:
- cultivating a eukaryotic cell comprising a nucleic acid encoding the polypeptide in a cultivation medium comprising a compound selected from the group consisting of trans-2-methyl 2-pentenoic acid, the broad-spectrum HDAC inhibitor Quisinostat, and the subtype-specific HDAC inhibitor Romidepsin, and
- recovering the polypeptide from the cell or the cultivation medium and thereby producing the polypeptide in a eukaryotic cell.
A method for the recombinant production of a polypeptide in a eukaryotic cell comprising the steps of:
- cultivating a eukaryotic cell comprising a nucleic acid encoding the polypeptide in a cultivation medium comprising a first and a second compound selected from the group of compounds consisting of trans-2-methyl 2-pentenoic acid, subtype-specific HDAC inhibitors, and canonical and non-canonical NFκB activators, and
- recovering the polypeptide from the cell or the cultivation medium and thereby producing the polypeptide in a eukaryotic cell.
Dependencies are inferred from the claim text.