Helper plasmid for transformation, method for producing transformant using the same, and transformation method

A transformation method, comprising a step of introducing into a host, one or several types of linear genome-introduced nucleic acid fragments each comprising a gene of interest to be introduced into a predetermined position on a genome, and a helper plasmid for transformation having a pair of homologous recombination sequences for incorporation of the linear genome-introduced nucleic acid fragments, wherein, in a state in which the linear genome-introduced nucleic acid fragments are incorporated into the helper plasmid for transformation, a pair of homologous recombination sequences for homologous recombination that takes place outside of the gene of interest and at the predetermined position on the genome, and a pair of endonuclease target sequences outside of the pair of homologous recombination sequences are disposed,

• wherein the gene of interest is expressed.

A helper plasmid for transformation, which is capable of incorporating into a genome, a linear genome-introduced nucleic acid fragment comprising a gene of interest to be introduced into a predetermined position on the genome, and which comprises a pair of homologous recombination sequences to be homologously recombined with the outside of the gene of interest in the linear genome-introduced nucleic acid fragment, and a pair of endonuclease target sequences disposed on the side opposite to the position into which the linear genome-introduced nucleic acid fragment is incorporated via the homologous recombination sequences.