Plants having increased tolerance to herbicides
AssigneeBASF SE
InventorsDoreen Schachtschabel, Eva Hollenbach, Mihiret Tekeste Sisay, Thomas Zierke, Danny Geerdink
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Abstract
Provided is a plant or plant part comprising a polynucleotide encoding a mutant TriA polypeptide, the expression of the polynucleotide confers to the plant or plant part tolerance to herbicides
Claims25
A plant or plant part comprising a polynucleotide encoding a mutated TriA polypeptide, the expression of said polynucleotide confers to the plant or plant part tolerance to herbicides.
A seed capable of germination into a plant comprising in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a mutated TriA polypeptide encoded by the polynucleotide, the expression of the mutated TriA polypeptide conferring to the plant tolerance to herbicides.
A plant cell of or capable of regenerating a plant comprising in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a mutated TriA polypeptide encoded by the polynucleotide, the expression of the mutated TriA polypeptide conferring to the plant tolerance to herbicides, wherein the plant cell comprises the polynucleotide operably linked to a promoter.
A plant cell comprising a polynucleotide operably linked to a promoter operable in a cell, the promoter capable of expressing a mutated TriA polypeptide encoded by the polynucleotide, the expression of the mutated TriA polypeptide conferring to the plant tolerance to herbicides.
A plant product prepared from a plant or plant part comprising in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a mutated TriA polypeptide encoded by the polynucleotide, the expression of the mutated TriA polypeptide conferring to the plant tolerance to herbicides.
A progeny or descendant plant derived from a plant comprising in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a mutated TriA polypeptide encoded by the polynucleotide, wherein the progeny or descendant plant comprises in at least some of its cells the polynucleotide operably linked to the promoter, the expression of the mutated TriA polypeptide conferring to the progeny or descendant plant tolerance to the herbicides.
A method for controlling weeds at a locus for growth of a plant, the method comprising:
- (a) applying an herbicide composition comprising herbicides to the locus;
- and (b) planting a seed at the locus, wherein the seed is capable of producing a plant that comprises in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a mutated TriA polypeptide encoded by the polynucleotide, the expression of the mutated TriA polypeptide conferring to the plant tolerance to herbicides.
A method of producing a plant having tolerance to herbicide, the method comprising regenerating a plant from a plant cell transformed with a recombinant polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a mutated TriA polypeptide encoded by the polynucleotide, the expression of the mutated TriA polypeptide conferring to the plant tolerance to herbicides.
A method of producing a progeny plant having tolerance to herbicide, the method comprising:
- crossing a first herbicide-tolerant plant with a second plant to produce a herbicide-tolerant progeny plant, wherein the first plant and the progeny plant comprise in at least some of their cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a mutated TriA polypeptide encoded by the polynucleotide, the expression of the mutated TriA polypeptide conferring to the plant tolerance to herbicides.
A plant or plant part comprising in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a mutated TriA polypeptide encoded by the polynucleotide, the expression of the mutated TriA polypeptide conferring to the plant tolerance to herbicides, wherein the plant or plant part further exhibits a second or third herbicide-tolerant trait.
An isolated and/or recombinantly produced and/or synthetic nucleic acid molecule comprising a nucleic acid molecule encoding a mutated TriA polypeptide selected from the group consisting of:
- (a) a nucleic acid molecule encoding a mutated TriA polypeptide comprising the sequence of SEQ ID NO:
- 2, or a variant, paralogue, orthologue or homolog thereof;
- (b) a nucleic acid molecule comprising the sequence of SEQ ID NO:
- 1, or a variant, paralogue, orthologue or homolog thereof;
- (c) a nucleic acid molecule, which, as a result of the degeneracy of the genetic code, can be derived from a TriA polypeptide sequence SEQ ID NO:
- 2, or a variant, paralogue, orthologue or homolog thereof, and confers increased herbicide tolerance or resistance, as compared to a corresponding, e.g. non-transformed, wild type plant cell, a plant or a part thereof;
- (d) a nucleic acid molecule having 30% or more identity, preferably 40%, 50%, 60%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99,5%, or more with the nucleic acid molecule sequence of a polynucleotide comprising the nucleic acid molecule of SEQ ID NO:
- 1, or a variant, paralogue, orthologue or homolog thereof, and confers increased herbicide tolerance or resistance, as compared to a corresponding, e.g. non-transformed, wild type plant cell, a plant or a part thereof;
- (e) a nucleic acid molecule encoding a mutated TriA polypeptide having 30% or more identity, preferably at least 40%, 50%, 60%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, 99,5% or more, with the amino acid sequence of the TriA polypeptide sequence SEQ ID NO:
- 2, and confers increased herbicide tolerance or resistance, as compared to a corresponding, e.g. non-transformed, wild type plant cell, a plant or a part thereof;
- nucleic acid molecule which hybridizes with a nucleic acid molecule of (a), (b), (c), (d) or (e) under stringent hybridization conditions and confers increased herbicide tolerance or resistance, as compared to a corresponding, e.g. non-transformed, wild type plant cell, a plant or a part thereof;
- wherein the amino acid sequence of the mutated TriA polypeptide differs from the wildtype amino acid sequence of a TriA polypeptide at one or more positions corresponding to the following positions of SEQ ID NO:2:
- 92, 93, 155, 157.
A plant product obtained from a plant or plant part thereof, wherein the plant or plant part comprises in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a mutated TriA polypeptide encoded by the polynucleotide, the expression of the mutated TriA polypeptide conferring to the plant tolerance to herbicides, wherein the plant or plant part further exhibits a second or third herbicide-tolerant trait.
Dependencies are inferred from the claim text.