Genome editing by pollen-mediated transformation

Abstract

Disclosed herein are methods of genome-editing transformation that delivers to the cell the RNA-guided endonucleases as well as the required guide RNA, without the need of tissue culture to regenerate the plant. Thus, methods as described herein enable any plant species to have its genome edited in a process that is simple and efficient. This process utilizes genome-editing pollen cells, and delivering the required components for the editing by combining them with cell-penetrating peptides.

Dependencies are inferred from the claim text.