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US 2020/0399635 A1Published application
Compositions and methods for molecular labeling
AssigneeBio-Rad Laboratories, Inc.
InventorsMichael L. Samuels, Jeffrey Charles Olson, Andrew Watson, Keith Brown, Darren R. Link
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Abstract
The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.
Claims23
-31. (canceled)
A method for analyzing at least one protein associated with a cell comprising:
- segregating a single cell in a fluid compartment;
- providing at least one barcoded binder to the fluid compartment, wherein said barcoded binder comprises an oligonucleotide comprising a barcode sequence;
- allowing the barcoded binder to bind to a protein in the single cell;
- sequencing the barcode of the oligonucleotide;
- and
- identifying the binder, thereby identifying the protein.
3 more elements
Dependencies are inferred from the claim text.