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US 2020/0399632 A1Published application
Sensor systems
AssigneeenEvolv, Inc.
InventorsMatthew R. DUNN, Kristin J. ADOLFSEN, Noah D. TAYLOR, James E. SPOONAMORE, Jay H. KONIECZKA, Caitlin D. ALLEN, Lindong WENG
Life of this patent
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Abstract
The present technology relates to methods and compositions that provide for improved detection of target molecules in, for example, bioengineering.
Claims141
A method for producing a population of engineered producer cells comprising:
- encapsulating each producer cell from a pool of genetically varied producer cells in a droplet to form a plurality of droplets encapsulating engineered producer cells;
- assaying the droplets for levels of a target molecule, wherein an engineered protein-based sensor provides a readout of the level of the desired target molecule produced by the producer cell through activation or repression of a reporter;
- isolating droplets with producer cells that produce desired levels of the target molecule;
- and,
- recovering the cells that produce desired levels of the target molecule to form the population of producer cells, wherein the population of producer cells is an enriched population that produce desired levels of the target molecule.
2 more elements
A method for producing a population of engineered producer cells comprising:
- transforming a pool of engineered producer cells with an engineered sensor plasmid, wherein the engineered sensor plasmid encodes an engineered protein sensor;
- encapsulating each producer cell from the pool of genetically varied producer cells in a droplet to form a plurality of droplets encapsulating engineered producer cells;
- assaying the droplets for levels of a target molecule, wherein an engineered protein-based sensor provides a readout of the level of the target molecule produced by the producer cell through activation or repression of a reporter;
- isolating the droplets with producer cells that produce desired levels of the target molecule;
- and,
- recovering the cells that produce desired levels of the target molecule to form the population of producer cells, wherein the population of producer cells is an enriched population that produce desired levels of the target molecule.
3 more elements
A method for producing a population of engineered producer cells comprising:
- encapsulating each producer cell from the pool of genetically varied producer cells in a droplet to form a plurality of droplets encapsulating engineered producer cells;
- merging each droplet containing the producer cell with a droplet encapsulating an engineered-protein based sensor cell, wherein the engineered sensor cell produces an engineered protein sensor;
- assaying the droplets for levels of a target molecule, wherein an engineered protein-based sensor provides a readout of the level of the desired target molecule produced by the producer cell through activation or repression of a reporter;
- sorting the merged droplets to isolate droplets containing producer cells that produce desired levels of the target molecule;
- and,
- recovering the cells that produce desired levels of the target molecule to form the population of producer cells, wherein the population of producer cells is an enriched population that produce desired levels of the target molecule.
3 more elements
The methods of any one of claims 63-78, wherein the DNA encoding the engineered protein-based sensor is integrated in the genome of the producer cell.
A method for producing a population of engineered producer cells comprising:
- transforming a pool of engineered producer cells with an engineered sensor plasmid, wherein the engineered sensor plasmid encodes an engineered protein sensor;
- encapsulating each producer cell from a pool of genetically varied producer cells in a droplet to form a plurality of droplets encapsulating engineered producer cells;
- wherein each droplet is surrounded by an immiscible continuous phase that comprises a fluorinated-based oil or emulsion;
- assaying the droplets for levels of a target molecule, wherein an engineered protein-based sensor provides a readout of the level of the desired target molecule produced by the producer cell through activation or repression of a reporter;
- isolating droplets with producer cells that produce desired levels of the target molecule;
- recovering the cells that produce desired levels of the target molecule to form the population of producer cells, wherein the population of producer cells is an enriched population that produce desired levels of the target molecule.
3 more elements
A method for producing a population of engineered producer cells comprising:
- encapsulating each producer cell from the pool of genetically varied producer cells in a droplet to form a plurality of droplets encapsulating engineered producer cells;
- wherein each droplet is:
- (a) surrounded by an immiscible continuous phase that comprises a fluorinated-based oil or emulsion, and
- (b) comprises an engineered sensor cell, wherein the engineered sensor cell produces an engineered protein sensor;
- assaying the droplets for levels of a target molecule, wherein an engineered protein-based sensor provides a readout of the level of the target molecule produced by the producer cell through activation or repression of a reporter;
- isolating the droplets with producer cells that produce desired levels of the target molecule;
- recovering the cells that produce desired levels of the target molecule to form the population of producer cells, wherein the population of producer cells is an enriched population that produce desired levels of the target molecule.
4 more elements
A method for producing a population of engineered producer cells comprising:
- encapsulating each producer cell from the pool of genetically varied producer cells in a droplet to form a plurality of droplets encapsulating engineered producer cells;
- wherein each droplet is surrounded by an immiscible continuous phase that comprises a fluorinated-based oil or emulsion;
- merging each droplet containing the producer cell with a droplet encapsulating an engineered-protein based sensor cell, wherein the engineered sensor cell produces an engineered protein sensor;
- assaying the droplets for levels of a target molecule, wherein an engineered protein-based sensor provides a readout of the level of the desired target molecule produced by the producer cell through activation or repression of a reporter;
- sorting the merged droplets to isolate droplets containing producer cells that produce desired levels of the target molecule;
- and,
- recovering the cells that produce desired levels of the target molecule to form the population of producer cells, wherein the population of producer cells is an enriched population that produce desired levels of the target molecule.
4 more elements
Dependencies are inferred from the claim text.