Methods and Compositions for Sample Analysis

A method of processing nucleic acid molecules, comprising:

• (a) providing a plurality of nucleic acid molecules, wherein said plurality of nucleic acid molecules comprises less than about 50 nanograms (ng) of nucleic acid molecules;
• (b) partitioning said plurality of nucleic acid molecules with a plurality of beads into a plurality of partitions, wherein a partition of said plurality of partitions comprises a nucleic acid molecule of said plurality of nucleic acid molecules and a bead of said plurality of beads, wherein said bead comprises a plurality of oligonucleotides coupled thereto, wherein an oligonucleotide of said plurality of oligonucleotides comprises a barcode sequence;
• and;
• (c) within said partition:
• (i) subjecting said bead to conditions sufficient to release said oligonucleotide from said bead;
• and
• (ii) using said oligonucleotide released from said bead and said nucleic acid molecule to generate a nucleic acid product, wherein said nucleic acid product comprises said barcode sequence, or complement thereof, and a sequence of said nucleic acid molecule, or complement thereof.