Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
AssigneeCodexis, Inc.
InventorsOscar Alvizo, Melissa Ann Mayo, Jeffrey C. Moore
Life of this patent
Estimating the term…
Abstract
The present application provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
Claims13
An engineered polypeptide comprising an amino acid sequence with at least 80% sequence identity to a reference sequence of SEQ ID NO:
- 8 and at least one substitution at a position selected from X9, X31, X33, X39, X43, X44, X48, X55, X56, X98, X166, X167, X168, X173, X198, X199, X203, X205, X206, X210, X213, X215, X216, X218, X224, X245, X251, X268, X269, X280, X285, X289, X290, X293, X330, X341, and X349, wherein the polypeptide has imine reductase activity as compared to SEQ ID NO:
- 8.
An engineered polypeptide comprising an amino acid sequence with at least 80% sequence identity to a reference sequence of SEQ ID NO:8 and at least two substitutions at positions selected from X9, X12, X31, X33, X37, X39, X43, X44, X46, X48, X55, X56, X65, X82, X93, X98, X108, X137, X138, X141, X142, X143, X153, X154, X155, X156, X159, X166, X167, X168, X173, X177, X178, X184, X185, X195, X197, X198, X199, X200, X201, X203, X205, X206, X210, X213, X215, X216, X218, X220, X221, X223, X224, X226, X234, X239, X242, X245, X251, X253, X256, X257, X259, X260, X261, X262, X263, X265, X266, X267, X268, X269, X272, X273, X274, X276, X277, X278, X279, X280, X281, X282, X283, X284, X285, X289, X290, X291, X292, X293, X294, X330, X341, and X349, wherein the polypeptide has imine reductase activity as compared to SEQ ID NO:8.
Dependencies are inferred from the claim text.