US 2020/0399614 A1 · Methods for Producing Mutant Bacteriophages for the Detection of Listeria

Abstract

Disclosed herein are methods for the production of mutant bacteriophages with altered host range. Additionally, disclosed herein are methods and systems for rapid detection of microorganisms such as Listeria spp. in a sample. A genetically modified bacteriophage is also disclosed which comprises an indicator gene in the late gene region. The specificity of the bacteriophage, such as Listeria-specific bacteriophage, allows detection of a specific microorganism, such as Listeria spp. and an indicator signal may be amplified to optimize assay sensitivity.

Claims13

Dependent claims

A method of producing a mutant bacteriophage with expanded host-range comprising:

(i) preparing a series of first co-culture mixtures of varying ratios comprising a host bacterial strain and a target-host bacterial strain;
(ii) adding a phage strain to each of the first co-culture mixtures;
(iii) incubating the first co-culture mixtures and the phage strain under bacterial culture conditions;
(iv) collecting a phage lysate from each of the plurality of first-co-cultures;
(v) pooling the phage lysates from each of the plurality of first co-cultures;
(vi) assaying phage lysates to determine if the bacteriophage host-range has expanded;
and
(vii) isolating a mutant phage with expanded host-range.

5 more elements

Dependencies are inferred from the claim text.

Facts

Application

16/905,607Filed 18 Jun 2020

Priority

21 Jun 2019

Published

24 Dec 2020

Term ends

Not estimatedThe source records don’t support an estimate.

Classification17 codes

A61P 31/04
Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics: antibacterial agents
C12N 7/00
Viruses
C07K 14/005
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from viruses
C12N 1/205
Bacteria; Culture media therefor: bacterial isolates

Methods for Producing Mutant Bacteriophages for the Detection of Listeria

Life of this patent

Methods for Producing Mutant Bacteriophages for the Detection of Listeria

Life of this patent

Abstract

Claims13