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US 2020/0399607 A1Published application
System and method for extraction and cryopreservation of bone marrow
AssigneeOssium Health, Inc.
InventorsErik John WOODS, Brian H. JOHNSTONE, Dongsheng GU, Aubrey Marie SHERRY, Kelsey Gwen MUSALL, John R. WOODS, James HARDIN, Alan HOOKS
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Abstract
Methods are provided for extracting bone marrow cells from bone obtained from deceased donors, for preparing the bone marrow for cryopreservation and for obtaining desired cells from cryopreserved and fresh bone marrow.
Claims23
A method for recovering vertebral bone adherent mesenchymal stromal/stem cells (vBA-MSC) from cadaver bone or cadaver bone fragments, the method comprising:
- (a) obtaining cadaver bone, cadaver bone fragments, or ground cadaver bone, optionally, preparing ground cadaver bone from the cadaver bone or cadaver bone fragments;
- (b) incubating the ground cadaver bone in a digestive solution comprising collagenase and/or neutral protease thereby obtaining a digested bone product;
- and
- (c) collecting the extracted bone marrow cells, thereby recovering the vBA-MSC.
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