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US 2020/0399605 A1Published application
System and method for extraction and cryopreservation of bone marrow
AssigneeOssium Health, Inc.
InventorsErik John WOODS, Brian H. JOHNSTONE, Dongsheng GU, Aubrey Marie SHERRY, Kelsey Gwen MUSALL, John R. WOODS, James HARDIN, Alan HOOKS
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Abstract
Methods are provided for extracting bone marrow cells from bone obtained from deceased donors, for preparing the bone marrow for cryopreservation and for obtaining desired cells from cryopreserved and fresh bone marrow.
Claims21
A method for high-yield recovery of stem cells from cadaver bone or cadaver bone fragments, the method comprising:
- (a) obtaining cadaver bone or cadaver bone fragments, optionally, processing the cadaver bone into cadaver bone fragments;
- (b) combining the cadaver bone fragments with a grinding medium comprising two or more of a nuclease, human serum albumin (HSA), heparin, an electrolyte medium, and a growth media, thereby obtaining grinding medium-treated cadaver bone fragments;
- (c) processing the medium-treated cadaver bone fragments to extract bone marrow cells;
- and
- (d) collecting the extracted bone marrow cells, thereby recovering the stem cells.
2 more elements
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Dependencies are inferred from the claim text.