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US 2020/0399604 A1Published application
System and method for extraction and cryopreservation of bone marrow
AssigneeOssium Health, Inc.
InventorsErik John WOODS, Brian H. JOHNSTONE, Dongsheng GU, Aubrey Marie SHERRY, Kelsey Gwen MUSALL, John R. WOODS, James HARDIN, Alan HOOKS
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Abstract
Methods are provided for extracting bone marrow cells from bone obtained from deceased donors, for preparing the bone marrow for cryopreservation and for obtaining desired cells from cryopreserved and fresh bone marrow.
Claims20
A method for high-yield recovery of stem cells from cadaver bone or cadaver bone fragments, the method comprising:
- (a) obtaining cadaver bone or cadaver bone fragments;
- (b) processing the bone or bone fragments to extract bone marrow cells;
- (c) combining the extracted bone marrow cells with a reduced density Ficoll solution having a density gradient of 1.063-1.052 g/ml;
- (d) centrifuging the extracted bone marrow cells, thereby separating out buffy coat cells comprising stem cells;
- and
- (e) harvesting the buffy coat cells, thereby recovering stem cells.
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