Method for generating cells of the t cell lineage
AssigneeSunnybrook Research Institute
InventorsJuan Carlos Zuniga-Pflucker, Mahmood Mohtashami, Ashton Trotman-Grant
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Abstract
A method of generating cells of the T cell lineage is provided comprising (a) culturing a sample comprising stem cells or progenitor cells with a Notch ligand conjugated to a suspension support and (b) isolating cells of the T cell lineage. In one embodiment, the cells of the T-cell lineage are progenitor T cells or mature T cells. Compositions, kits and uses thereof are also provided.
Claims27
A method of generating a cell of the T cell lineage comprising (a) culturing a sample comprising stem cells or progenitor cells with a Notch ligand conjugated to a suspension support and (b) isolating cells of the T cell lineage.
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A suspension Notch ligand comprising (a) a Notch ligand and (b) a suspension support, optionally a microbead, wherein the Notch ligand is conjugated to the suspension support.
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A method of treating a subject having a condition requiring an increase in the number of T cells comprising:
- (i) generating cells of the T cell lineage comprising (a) culturing a sample comprising stem cells or progenitor cells with a Notch ligand conjugated to a suspension support and (b) isolating cells of the T cell lineage, and
- (ii) administering an effective amount of the cells of the T cell lineage to the subject.
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Dependencies are inferred from the claim text.