Method for obtaining a spermatozoid cell population with improved fitness

A spermatozoid cell population enriched in CD10− spermatozoids.

An in vitro method for obtaining an embryo comprising:

• (i) contacting a starting spermatozoid population with a CD10-binding agent,
• (ii) isolating spermatozoids from the starting population which do not bind to the CD10-binding agent thereby obtaining a spermatozoid cell population enriched in CD10− spermatozoids and
• (iii) fertilizing an ovum using a CD10− enriched spermatozoid cell population.

An in vitro method for obtaining an embryo comprising:

• (i) contacting a starting spermatozoid population with a CD10-binding agent,
• (ii) isolating spermatozoids from the starting population which do not bind to the CD10-binding agent thereby obtaining a spermatozoid cell population enriched in CD10− spermatozoids and
• (iii) injecting into an ovum an spermatozoid from the CD10− enriched spermatozoid cell population obtained in step (ii).

An insemination method comprising:

• (i) contacting a starting spermatozoid population with a CD10-binding agent,
• (ii) isolating spermatozoids from the starting population which do not bind to the CD10-binding agent thereby obtaining a spermatozoid cell population enriched in CD10− spermatozoids and
• (iii) administering into the reproductive tract of a female subject the spermatozoid cell population obtained in step (ii).

Use of a CD10-binding agent for the preparation of a spermatozoid cell population enriched in CD10− spermatozoids.

Use of a CD10-binding agent for the preparation of a spermatozoid cell population having a high degree of DNA integrity.

Use of a CD10-binding agent for the preparation of a spermatozoid cell population having a low level of aneuploidies.

A method for determining the fitness of, the DNA integrity of or the number of aneuplodies in a human sperm population for fertilization which comprises determining the content of CD10− spermatozoids in a said population, wherein increased levels of CD10− spermatozoids in the population with respect to a reference value are indicative that the human sperm is adequate for fertilization, that the sperm population shows a high degree of DNA integrity or a low level of aneuploidies.