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US 2020/0399587 A1Published application
Culture medium for improving development of bovine in vitro fertilized embryos and cloned embryos
AssigneeNortheast A&F University
InventorsJingcheng ZHANG, YUEMENG HUANG, MIN ZHANG, DEBAO WANG, YONG WANG, YONG ZHANG
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Abstract
A culture medium for improving the development of bovine in vitro fertilized embryos and cloned embryos includes a SOF basal medium, progesterone, vitamin E, L-carnitine, FGF, corticosterone, citrulline, EGF, IGF and LIF.
Claims9
A culture medium for improving development of bovine in vitro fertilized embryos and cloned embryos, comprising:
- a synthetic oviduct fluid (SOF) basal medium, citrulline, progesterone, vitamin E, L-carnitine, fibroblast growth factor (FGF), corticosterone, epidermal growth factor (EGF), insulin growth factor (IGF) and leukemia inhibitory factor (LIF).
The culture method of claim 9, wherein the culture of the bovine in vitro fertilized embryos or the cloned embryos is performed at 38.5° C., 5% CO2 and 7% O2 under saturated humidity.
Dependencies are inferred from the claim text.