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US 2020/0399579 A1Published application
Flow through electroporation modules and instrumentation
AssigneeInscripta, Inc.
InventorsNathan Bade, Jorge Bernate, Phillip Belgrader, Don Masquelier
Life of this patent
Estimating the term…
Abstract
The present disclosure provides a flow-through electroporation device configured for use in an automated multi-module cell processing environment and configured to decrease cell processing time and the risk of clogging.
Claims20
A flow-through electroporation (FTEP) device for introducing an exogenous material into cells in a fluid, the FTEP device comprising:
- a. an inlet and ant inlet channel for receiving a fluid comprising cells and/or exogenous material into the FTEP device;
- b. an outlet and an outlet channel for removing a fluid comprising transformed cells and exogenous material from the FTEP device;
- c. a flow channel intersecting and positioned between the inlet channel and the outlet channel, wherein the flow channel has, moving from the inlet channel toward the outlet channel, an inlet-filter region, an inlet-proximal region, a central region, an outlet-proximal region, and an outlet-filter region;
- d. an inlet filter comprising filter elements disposed in the inlet-filter region of the flow channel and an outlet filter comprising filter elements disposed in the outlet-filter region of the flow channel;
- e. a plurality of parallel-configured obstructions defining flow lanes disposed within the central region of the flow channel;
- and
- f. a first and a second electrode positioned in electrode channels, wherein the first electrode is positioned in the inlet proximal region of the flow channel and the second electrode is positioned in the outlet proximal region of the flow channel;
- wherein the electrodes are positioned perpendicularly to the flow channel, are in fluid and electrical communication with fluid in the flow channel, and wherein the electrodes apply one or more electric pulses to the cells in the fluid as they pass through the flow channel, thereby introducing exogenous material into the cells in the fluid.
5 more elements
The FTEP device of claim 16, wherein the pressure in the FTEP is from 2-10 psi.
A flow-through electroporation (FTEP) device for introducing an exogenous material into cells in a fluid, the device comprising:
- a. an inlet and an inlet channel for receiving a fluid comprising cells and/or exogenous material into the FTEP device;
- b. an outlet and an outlet channel for removing a fluid comprising transformed cells and exogenous material from the FTEP device;
- c. a flow channel intersecting and positioned between the inlet channel and the outlet channel, wherein the flow channel has, moving from the inlet channel to the outlet channel, an inlet-filter region, an inlet-proximal region, a central region, an outlet-proximal region, and an outlet-filter region;
- d. an inlet filter comprising filter elements disposed in the inlet-filter region of the flow channel, wherein the inlet filter comprises triangular-, square-, rectangular-, pentagonal-, hexagonal-, oval-, or elliptical-shaped elements;
- e. a plurality of obstructions defining flow paths disposed within the central region of the flow channel, wherein the number of obstructions increases in density from the inlet proximal region of the flow channel to a central portion of the central region, and wherein the number of obstructions decreases in density from the central portion of the central region to the outlet proximal region of the flow channel;
- and
- f. a first and a second electrode positioned in electrode channels, wherein the first electrode is positioned in the inlet-proximal region of the flow channel and the second electrode is positioned in the outlet-proximal region of the flow channel;
- wherein the electrodes are positioned perpendicularly to the flow channel from a top of the FTEP device, are in fluid and electrical communication with fluid in the flow channel, and wherein the electrodes apply one or more electric pulses to the cells in the fluid as they pass through the flow channel, thereby introducing exogenous material into the cells in the fluid.
5 more elements
Dependencies are inferred from the claim text.