Devices, Methods, and Systems for Electroporation
AssigneeCyteQuest, Inc.
InventorsTom Corso, Harold G. Craighead
Life of this patent
Estimating the term…
Abstract
Disclosed are a system, device, and method for the electroporation of cells. Systems, devices and methods for electroporation of living cells and the introduction of selected molecules into the cells utilizes a fluidic system where living cells and biologically active molecules flow through a channel that exposes them to electric fields, causing the molecules to be transferred across the cell membrane. The methods are particularly well suited for the introduction of DNA, RNA, drug compounds, and other biologically active molecules into living cells for use in cell-based therapies.
Claims26
An electroporation device comprising
- a first-side support having at least one first-side electrode disposed at a region of an inner surface of the first-side support;
- a second-side support having at least one second-side electrode disposed at a region of an inner surface of the second-side support, wherein the at least one second-side electrode is a counterpart electrode of the at least one first-side electrode;
- and
- a fluid channel, having at least a portion of it between the first-side support and the second-side support, and having at least one fluid input and at least one fluid output, wherein the fluid channel allows fluid to flow continuously in at least one fluid stream toward the at least one fluid output,
- wherein the at least one first-side electrode and the at least one second-side electrode are positioned in the electroporation device to allow modulating the electric field as a predetermined function of time and position in at least one part of the fluid channel between the first-side support and the second-side support.
10-21. (canceled)
An electroporation device comprising
- a first-side support having at least one first-side electrode disposed at a region of an inner surface of the first-side support;
- a second-side support having at least one second-side electrode disposed at a region of an inner surface of the second-side support, wherein the at least one second-side electrode is a counterpart electrode of the at least one first-side electrode;
- and
- a fluid channel, having at least a portion of it between the first-side support and the second-side support, and having at least two fluid inputs and at least one fluid output, wherein the fluid channel allows fluid to flow continuously in at least two fluid streams toward the at least one fluid output,
- wherein the fluid channel allows modulating the flow rate, chemical composition, or both the flow rate and chemical composition in the at least two fluid streams as a predetermined function of time, position, or both time and position.
28-31. (canceled)
A system, comprising
- an electroporation device, comprising
- a first-side support having at least one first-side electrode disposed at a region of an inner surface of the first-side support;
- a second-side support having at least one second-side electrode disposed at a region of an inner surface of the second-side support, wherein the at least one second-side electrode is a counterpart electrode of the at least one first-side electrode;
- and
- a fluid channel, having at least a portion of it between the first-side support and the second-side support, and having at least one fluid input and at least one fluid output, wherein the fluid channel allows fluid to flow continuously in at least one fluid stream toward the at least one fluid output, wherein the at least one first-side electrode and the at least one second-side electrode are positioned in the electroporation device to allow modulating the electric field as a predetermined function of time and position in at least one part of the fluid channel between the first-side support and the second-side support;
- and
- a fluid-delivery apparatus coupled to the electroporation device.
33-34. (canceled)
36-37. (canceled)
40-42. (canceled)
44-57. (canceled)
Dependencies are inferred from the claim text.