US 2020/0399349 A1 · ANTIBODY AGAINST CHIKUNGUNYA VIRUS OR ANTIGEN-BINDING FRAGMENT OF THE ANTIBODY, AND USE OF THE SAME

Abstract

The present invention provides a new antibody against ECSA type Chikungunya virus, WA type Chikungunya virus, and Asian type Chikungunya virus or an antigen-binding fragment of the antibody. The antibody against Chikungunya virus or the antigen-binding fragment of the antibody of the present invention includes a heavy chain variable region or a heavy chain (1), (2), or (3) and a light chain variable region or a light chain (4).

An antibody against Chikungunya virus or an antigen-binding fragment of the antibody, comprising the following heavy chain variable region (1), (2), or (3) and the following light chain variable region (4):

(1) a heavy chain variable region comprising heavy chain complementarity determining regions (CDRH)1, CDRH2, and CDRH3:
CDRH1 is a polypeptide comprising the following amino acid sequence (H1-A),
CDRH2 is a polypeptide comprising the following amino acid sequence (H2-A), and
CDRH3 is a polypeptide comprising the following amino acid sequence (H3-A), wherein
(H1-A) the following amino acid sequence (H1-A1), (H1-A2), or (H1-A3):
(H1-A1) an amino acid sequence of SEQ ID NO:
1 (GYTFTSYW),
(H1-A2) an amino acid sequence having 80% or more identity with respect to the amino acid sequence of SEQ ID NO:
1, and
(H1-A3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted and/or added in the amino acid sequence of SEQ ID NO:
1,
(H2-A) the following amino acid sequence (H2-A1), (H2-A2), or (H2-A3):
(H2-A1) an amino acid sequence of SEQ ID NO:
2 (IYPGDGDTRYT),
(H2-A2) an amino acid sequence having 80% or more identity with respect to the amino acid sequence of SEQ ID NO:
2, and
(H2-A3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted and/or added in the amino acid sequence of SEQ ID NO:
2,
(H3-A) the following amino acid sequence (H3-A1), (H3-A2), or (H3-A3):
(H3-A1) an amino acid sequence of SEQ ID NO:
3 (SYDPFDY),
(H3-A2) an amino acid sequence having 80% or more identity with respect to the amino acid sequence of SEQ ID NO:
3, and
(H3-A3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted and/or added in the amino acid sequence of SEQ ID NO:
3,
(2) a heavy chain variable region comprising CDRH1, CDRH2, and CDRH3:
CDRH1 is a polypeptide comprising the following amino acid sequence (H1-B),
CDRH2 is a polypeptide comprising the following amino acid sequence (H2-B), and
CDRH3 is a polypeptide comprising the following amino acid sequence (H3-B), wherein
(H1-B) the following amino acid sequence (H1-B1), (H1-B2), or (H1-B3):
(H1-B1) an amino acid sequence of SEQ ID NO:
4 (GYAFSTSW),
(H1-B2) an amino acid sequence having 80% or more identity with respect to the amino acid sequence of SEQ ID NO:
4, and
(H1-B3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted and/or added in the amino acid sequence of SEQ ID NO:
4,
(H2-B) the following amino acid sequence (H2-B1), (H2-B2), or (H2-B3):
(H2-B1) an amino acid sequence of SEQ ID NO:
5 (IYPGDGDT),
(H2-B2) an amino acid sequence having 80% or more identity with respect to the amino acid sequence of SEQ ID NO:
5, and
(H2-B3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted and/or added in the amino acid sequence of SEQ ID NO:
5,
(H3-B) the following amino acid sequence (H3-B1), (H3-B2), or (H3-B3):
(H3-B1) an amino acid sequence of SEQ ID NO:
6 (SNDGYYVGY),
(H3-B2) an amino acid sequence having 80% or more identity with respect to the amino acid sequence of SEQ ID NO:
6, and
(H3-B3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted and/or added in the amino acid sequence of SEQ ID NO:
6,
(3) a heavy chain variable region comprising CDRH1, CDRH2, and CDRH3:
CDRH1 is a polypeptide comprising the following amino acid sequence (H1-C),
CDRH2 is a polypeptide comprising the following amino acid sequence (H2-C),
CDRH3 is a polypeptide comprising the following amino acid sequence (H3-C), wherein
(H1-C) the following amino acid sequence (H1-C1), (H1-C2), or (H1-C3):
(H1-C1) an amino acid sequence of SEQ ID NO:
7 (GYTFTSYY),
(H1-C2) an amino acid sequence having 80% or more identity with respect to the amino acid sequence of SEQ ID NO:
7, and
(H1-C3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted and/or added in the amino acid sequence of SEQ ID NO:
7,
(H2-C) the following amino acid sequence (H2-C1), (H2-C2), or (H2-C3):
(H2-C1) an amino acid sequence of SEQ ID NO:
8 (INPSNGGT),
(H2-C2) an amino acid sequence having 80% or more identity with respect to the amino acid sequence of SEQ ID NO:
8, and
(H2-C3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted and/or added in the amino acid sequence of SEQ ID NO:
8,
(H3-C) the following amino acid sequence (H3-C1), (H3-C2), or (H3-C3):
(H3-C1) an amino acid sequence of SEQ ID NO:
9 (GYYGNPFFAY),
(H3-C2) an amino acid sequence having 80% or more identity with respect to the amino acid sequence of SEQ ID NO:
9, and
(H3-C3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted and/or added in the amino acid sequence of SEQ ID NO:
9,
(4) a light chain variable region comprising light chain complementarity determining regions (CDRL)1, CDRL2, and CDRL3:
CDRL1 is a polypeptide comprising the following amino acid sequence (L1-A),
CDRL2 is a polypeptide comprising the following amino acid sequence (L2-A),
CDRL3 is a polypeptide comprising the following amino acid sequence (L3-A), wherein
(L1-A) the following amino acid sequence (L1-A1), (L1-A2), or (L1-A3):
(L1-A1) an amino acid sequence of SEQ ID NO:
10 (ENVVTY),
(L1-A2) an amino acid sequence having 80% or more identity with respect to the amino acid sequence of SEQ ID NO:
10, and
(L1-A3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted and/or added in the amino acid sequence of SEQ ID NO:
10,
(L2-A) the following amino acid sequence (L2-A1), (L2-A2), or (L2-A3):
(L2-A1) an amino acid sequence of SEQ ID NO:
11 (GAS),
(L2-A2) an amino acid sequence having 80% or more identity with respect to the amino acid sequence of SEQ ID NO:
11, and
(L2-A3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted and/or added in the amino acid sequence of SEQ ID NO:
11,
(L3-A) the following amino acid sequence (L3-A1), (L3-A2), or (L3-A3):
(L3-A1) an amino acid sequence of SEQ ID NO:
12 (GQGYSYPYT),
(L3-A2) an amino acid sequence having 80% or more identity with respect to the amino acid sequence of SEQ ID NO:
12, and
(L3-A3) an amino acid sequence in which one or several amino acids are deleted, substituted, inserted and/or added in the amino acid sequence of SEQ ID NO:
12.

97 more elements

Antibody against chikungunya virus or antigen-binding fragment of the antibody, and use of the same

Life of this patent

Antibody against chikungunya virus or antigen-binding fragment of the antibody, and use of the same

Life of this patent

Abstract

Claims8