IL-15 Conjugates and Uses Thereof
AssigneeSynthorx, Inc.
InventorsCarolina E. CAFFARO, Jerod PTACIN, Marcos MILLA
Life of this patent
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Abstract
Disclosed herein are interleukin (IL) 15 conjugates and use in the treatment of one or more indications. Also described herein include pharmaceutical compositions and kits comprising one or more of IL-15 conjugates.
Claims93
An isolated and modified interleukin 15 (IL-15) polypeptide comprising at least one unnatural amino acid at a position on the polypeptide that reduces binding between the modified IL-15 polypeptide and interleukin 15 receptor α (IL-15Rα) but retains significant binding with interleukin 15 receptor βγ (IL-15Rβγ) signaling complex to form an IL-15/IL-15Rβγ complex, wherein the reduced binding to IL-15Rα is compared to binding between a wild-type IL-15 polypeptide and IL-15Rα.
An isolated and modified interleukin 15 (IL-15) polypeptide comprising at least one unnatural amino acid at a position on the polypeptide that enhances half-life, wherein the enhanced half-life is compared to a half-life of a wild-type IL-15 protein.
An isolated and modified interleukin 15 (IL-15) polypeptide comprising at least one unnatural amino acid at a position on the polypeptide that reduces binding between the modified IL-15 polypeptide and interleukin 15 receptor α (IL-15Rα) but retains significant signaling potency with interleukin 15 receptor βγ (IL-15Rβγ) signaling complex, wherein the reduced binding to IL-15Rα is compared to binding between a wild-type IL-15 polypeptide and IL-15Rα.
An isolated and modified interleukin 15 (IL-15) polypeptide comprising at least one unnatural amino acid, wherein the at least one unnatural amino acid is at a residue position that selectively decreases the binding affinity of the modified IL-15 polypeptide with interleukin 2/interleukin 15 receptor β (IL-2/IL-15Rβ), interleukin 15 receptor γ (IL-15Rγ), or a combination thereof, but does not affect the interaction with the interleukin 15 receptor α (IL-15R α).
An interleukin 15 (IL-15) conjugate comprising:
- an isolated and purified IL-15 polypeptide;
- and
- a conjugating moiety that binds to the isolated and purified IL-15 polypeptide at an amino acid position selected from N4, E46, D61, E64, N65, I68 and L69, wherein the numbering of the amino acid residues corresponds to SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.