Methods and compositions for antibody-evading virus vectors
AssigneesThe University of North Carolina at Chapel Hill, University of Florida Research Foundation, Inc.
InventorsAravind Asokan, Mavis Agbandje-McKenna, Long Ping Victor Tse, Brittney Gurda
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Abstract
The present invention provides AAV capsid proteins comprising a modification in the amino acid sequence and virus vectors comprising the modified AAV capsid protein. The invention also provides methods of administering the virus vectors and virus capsids of the invention to a cell or to a subject in vivo.
Claims25
1-207. (canceled)
A recombinant adeno associated virus (AAV) vector comprising a capsid protein and a nucleic acid, wherein the capsid protein comprises one or more of the following substitutions, wherein the amino acids are numbered according to the amino acid sequence of SEQ ID NO:1:
- (a) a substitution of amino acids corresponding to amino acids 456 to 459 with the amino acid sequence SERR (SEQ ID NO:26);
- (b) a substitution of amino acids corresponding to amino acids 492 to 499 with the amino acid sequence TPGGNATR (SEQ ID NO:485);
- and
- (c) a substitution of amino acids corresponding to amino acids 588 to 597 with the amino acid sequence DLDPKATEVE (SEQ ID NO:487),
- wherein the capsid protein with the substitutions of one or more of (a)-(c) has an amino acid sequence that is at least about 90% identical to the amino acid sequence of SEQ ID NO:1.
Dependencies are inferred from the claim text.