Methods for sequencing biomolecules

Abstract

A system and method for providing sequencing of biomolecules, which can be used for differential analysis of a test sample from a normal sample. Methods can involve steps of providing a mapped sequence file of each of a pilot test sample and a pilot normal sample, wherein each sequence file has a pilot number of reads; calculating, by a processor, a first test-normal genomic comparison pilot view from the sequence files of the pilot test sample and the pilot normal sample, wherein the first pilot view distinguishes pilot test sample data from pilot normal sample data based on at least one genomic parameter; calculating, by the processor, for each sequence file a downsampled sequence file having a reduced pilot number of reads; calculating, by the processor, a second test-normal genomic comparison pilot view from the downsampled sequence files of the pilot test sample and the pilot normal sample, wherein the second pilot view distinguishes the pilot test sample data from the pilot normal sample data based on the at least one genomic parameter; repeating the downsampling steps for determining the fewest pilot number of reads required for calculating a test-normal genomic comparison view that distinguishes the pilot test sample data from the pilot normal sample data based on the at least one genomic parameter; sequencing biomolecules of the test sample and the normal sample using a number of reads equal to the fewest pilot number of reads; calculating, by the processor, a test-normal genomic comparison view for displaying the differential analysis based on the at least one genomic parameter.

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