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US 2020/0393447 A1Published application
Biosensors for measuring cell signaling in stressed and healthy cells
AssigneeMontana Molecular LLC
InventorsTHOMAS HUGHES, KEVIN HARLEN
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Abstract
Disclosed herein are nucleic acids comprising reporter constructs for detecting cellular signaling changes in a stressed cell. Also provided are methods for detecting cellular signaling changes in cells undergoing stress, as well as vectors and cells comprising nucleic acids comprising reporter constructs for detecting cellular signaling changes in a stressed cell.
Claims33
A nucleic acid comprising from 5′ to 3′;
- a) a first open reading frame encoding a first reporter protein;
- b) a linker sequence comprising an intron that is spliced by inositol-requiring enzyme 1 (IRE1) when transcribed to mRNA;
- and
- c) a second open reading frame encoding a second reporter protein, wherein the first and second open reading frames are out of frame from each other.
1 more element
A nucleic acid comprising from 5′ to 3′;
- a) a first exon of a reporter protein;
- b) a linker sequence comprising an intron that is spliced by inositol-requiring enzyme 1 (IRE1);
- and
- c) a second exon of the reporter protein, wherein the second exon is in a different reading frame from the first exon,
- wherein splicing of the intron in mRNA by IRE1 places the second exon in the same reading frame as the first exon.
2 more elements
Dependencies are inferred from the claim text.