Device for performing electrical measurements
AssigneeMimetas B.V.
InventorsPaul VULTO, Sebastian Johannes TRIETSCH, Arnaund Yannick Michel NICOLAS, Frederik Mathijs SCHAVEMAKER
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AssigneeMimetas B.V.
InventorsPaul VULTO, Sebastian Johannes TRIETSCH, Arnaund Yannick Michel NICOLAS, Frederik Mathijs SCHAVEMAKER
Estimating the term…
A device for performing electrical measurements, for example electrical activity across a layer of epithelial cells are disclosed. The device comprises a cassette having first and second surfaces, the cassette configured to engage with a microtiter plate and comprising a plurality of electrodes extending from the first surface in the direction of the microtiter plate when the cassette is engaged with the microtiter plate; and a housing detachably attached to the second surface of the cassette, comprising one or more heat management elements, and a processor comprising a data acquisition module electrically connected to the electrodes and a data processing module. A method of in vitro method for measuring electrical properties of cells cultured in a microfluidic device, for example using the device is also described.
A device for performing electrical measurements, comprising:
A device according to any one of the preceding claims, wherein the processor is configured to perform AC frequency sweeps, preferably in a range of from 1 Hz to 100 Mhz, more preferably in a range of from 10 Hz to 10 MHz, preferably wherein the range of the frequency sweep and frequency of data acquisition by the data acquisition module is adaptable in a manual, automated or iterative fashion, preferably optimized to the characteristics of the system being measured.
A device according to any one of the preceding claims, wherein the plurality of electrodes are disposed in a predetermined configuration corresponding to the configuration of at least two or more wells of a microtiter plate;
A device according to any one of the preceding claims, wherein two or more electrodes are immersed into a single well, thereby allowing for a 4-point electrical measurement which enables better electrical characterization of the electrical circuit and/or device under test (DUT).
A device according to any one of the preceding claims, further comprising one or more clamping mechanisms to ensure accurate and repeatable positioning of the cassette to the housing and/or accurate and repeatable positioning of the electrodes within the wells of the microtiter plate.
A device according to any one of the preceding claims, wherein the electrode material comprises a biocompatible material, wherein the electrode material preferably is platinum, gold plated brass, gold plated stainless steel or stainless steel.
A device according to any one of the preceding claims, wherein the electrodes comprise one or more of a silver chloride electrode, an ion selective electrode, or a biofunctionalized electrode.
A device according to any one of the preceding claims, wherein the one or more heat management elements comprises elements thermally decoupling the cassette from the housing, such as insulating layers or spacers between the housing and cassette.
A device according to any one of the preceding claims, wherein the one or more heat management elements includes passive or active heat conduits to move heat away from the cassette, wherein the one or more heat management elements comprise one or more of radiating surfaces, cooling fins, liquid cooling, Peltier modules, air ducts, or fans improving airflow through or around the device, or any combination thereof.
A device according to any one of the preceding claims, further comprising a base configured to receive a microtiter plate and to detachably engage with the cassette and/or housing.
A device according to any one of the preceding claims, wherein the total footprint of the device is less than twice the footprint of the titerplate, preferably less than 1.5 times the footprint of the titerplate, thereby allowing interaction of the titerplate with external equipment while engaged in the device.
A device according to one of the preceding claims, wherein the cassette comprises at least 80 electrodes, more preferably 96 electrodes, more preferably 128 electrodes, more preferably 248 electrodes.
An in vitro method for measuring electrical properties of cells cultured in a microfluidic device, the method comprising the steps of
Dependencies are inferred from the claim text.