Flagellin-based agents and uses including effective vaccination

A method of vaccinating a subject against a disorder, comprising administering an effective amount of a vaccine comprising:

• (a) an adjuvant comprising:
• a flagellin-based agent comprising an amino acid sequence having at least 95% identity with one of SEQ ID NO:
• 35, 71, and 150, and
• an aluminum gel or salt,
• wherein the ratio (w/w) of flagellin-based agent to aluminum gel or salt is about 1:500 or less and
• (b) an antigen which stimulates protective immunity against the disorder,
• wherein the antigen is a constituent of an infectious agent selected from a live and attenuated, killed, inactivated, and toxoid infectious agent;
• and wherein the disorder is selected from infectious diseases, cancers, and allergies.