Microorganisms with increased photosynthetic capacity
AssigneeLumen Bioscience, Inc.
InventorsJames ROBERTS, Damian CARRIERI, Mark HEINNICKEL
Life of this patent
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Abstract
Microorganisms with increased photosynthetic capacity are described. Increased photosynthetic capacity is achieved by down-regulating activity of the RpaB pathway. The microorganisms include Cyanobacteria, including genetically-modified Cyanobacteria.
Claims31
A genetically-modified Cyanobacteria comprising an exogenous nucleotide sequence comprising a SrrA sequence that expresses a SrrA decoy under the control of a promoter that results in down-regulated RpaB pathway activity in the genetically-modified Cyanobacteria as compared to a Cyanobacteria of the same species without the genetic modification.
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The genetically-modified Cyanobacteria of claim wherein the SrrA decoy is selected from:
- (a) an N-terminal fragment SrrA comprising a phosphor-receiver domain but no DNA binding domain or a non-functional DNA binding domain;
- (b) an N-terminal fragment of wild type SrrA including Asp64 or a conservative substitution thereof;
- (c) N-SrrA;
- or
- (d) an N-SrrA variant that maintains Asp64 or a conservative substitution thereof.
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A method of increasing photosynthetic capacity and/or biomass accumulation of a Cyanobacteria comprising modifying the Cyanobacteria to reduce RpaB pathway activity within the Cyanobacteria as compared to a Cyanobacteria of the same species without the modification or as compared to a wild type Cyanobacteria of the same species, wherein the modifying comprises inserting an exogenous nucleotide sequence that expresses an SrrA decoy under the control of a promoter into the Cyanobacteria.
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A method of increasing photoautotrophic growth of a Cyanobacteria comprising:
- modifying the Cyanobacteria to reduce RpaB pathway activity within the Cyanobacteria as compared to a Cyanobacteria of the same species without the modification or as compared to a wild type Cyanobacteria of the same species
- and culturing the modified Cyanobacteria in a liquid culture,
- thereby increasing photoautotrophic growth of the Cyanobacteria as compared to the Cyanobacteria of the same species without the modification or as compared to the wild type Cyanobacteria of the same species, wherein the modifying comprises inserting an exogenous nucleotide sequence that expresses an SrrA decoy under the control of a promoter into the Cyanobacteria.
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Dependencies are inferred from the claim text.