Solubilization of antigen components for removal from tissues
AssigneeThe Regents of the University of California
InventorsLeigh G. Griffiths, Angeliki Papalamprou, Maelene L. Wong
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Abstract
The present invention relates to methods for removing antigens from tissues by sequentially destabilizing and/or depolymerizing cytoskeletal components and removing and/or reducing water-soluble antigens and lipid-soluble antigens. The invention further relates to tissue scaffolding and decellularized extracellular matrix produced by such methods.
Claims20
A decellularized extracellular matrix (ECM) scaffold depleted of antigens comprising ECM structure and ECM tensile strength that is substantially the same as the ECM prior to antigen removal, wherein the ECM is substantially free of endogenous antigens, wherein at least 60% of the lipid-soluble antigens are removed from the ECM, wherein the glycosaminoglycan (GAG) content is substantially decreased in comparison to the ECM prior to antigen removal, and wherein the ECM is repopulated with live cells.
A decellularized extracellular matrix (ECM) scaffold depleted of antigens comprising:
- i) ECM tensile strength, ECM collagen and ECM elastin that is substantially the same as the ECM prior to antigen removal, wherein the ECM is repopulated with live cells;
- ii) substantially reduced glycosaminoglycan (GAG) content in comparison to a native ECM prior to antigen removal;
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- iii) at least 60% reduction of lipid-soluble antigens in comparison to a native tissue control.
Dependencies are inferred from the claim text.