Novel aav mediated influenza vaccines

A non-replicating recombinant adeno-associated associated virus (rAAV) having an AAV capsid having packaged therein a vector genome which comprises AAV inverted terminal repeat sequences and at least one nucleic acid sequence encoding an immunoglobulin region (a), (b), (c) and (d), wherein each of immunoglobulin regions (a), (b), (c) and (d) is expressed from the rAAV, wherein

• (a) is a first immunoglobulin region having the amino acid sequence of SEQ ID NO:1:
• (Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Ile Ser Ile Phe Asp Ile Tyr Ala Met Asp Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val Ala Val Ser Phe Arg Asp Gly Ser Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys His Val Ser Leu Tyr Arg Asp Pro Leu Gly Val Ala Gly Gly Ile Gly Val Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser);
• (b) a second immunoglobulin region having an amino acid sequence of SEQ ID NO:
• 2:
• (Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Tyr Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val Ala Ala Ile Asn Ala Leu Gly Thr Arg Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Thr Ala Gln Gly Gln Trp Arg Ala Ala Pro Val Ala Val Ala Ala Glu Tyr Glu Phe Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser);
• (c) a third immunoglobulin region having an amino acid sequence of SEQ ID NO:
• 3:
• (Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Leu Glu Asn Lys Ala Ile Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Gly Val Leu Cys Ile Ser Lys Ser Gly Ser Trp Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Arg Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala Thr Thr Thr Ala Gly Gly Gly Leu Cys Trp Asp Gly Thr Thr Phe Ser Arg Leu Ala Ser Ser Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser);
• and
• (d) a fourth immunoglobulin region having an amino acid sequence of SEQ ID NO:4:
• (Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Thr Ser Trp Met Tyr Trp Leu Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ser Val Ile Asn Thr Asp Gly Gly Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Lys Asp Trp Gly Gly Pro Glu Pro Thr Arg Gly Gln Gly Thr Leu Val Thr Val Ser Ser).