Targeted extracellular vesicles comprising membrane proteins with engineered glycosylation sites
AssigneeNorthwestern University
InventorsJoshua N. Leonard, Michelle E. Hung
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Abstract
Disclosed are extracellular vesicles comprising an engineered targeting protein for targeting the extracellular vesicles to target cells. The targeting protein is a fusion protein that includes a ligand, an engineered glycosylation site, and an exosome-targeting domain. Exemplary extracellular vesicles may include but are not limited to exosomes.
Claims19
1-22. (canceled)
A method of loading an exosome with a cargo nucleic acid, comprising expressing within an exosome-producing cell (i) a fusion protein comprising a nucleic acid-binding domain and an exosome-targeting domain, and (ii) a cargo nucleic acid comprising a binding motif;
- wherein the binding motif of the cargo nucleic acid binds to the nucleic acid-binding domain of the fusion protein and the exosome-targeting domain of the fusion protein localizes to an exosome, such that the cargo nucleic acid is loaded into the exosome when the fusion protein and cargo nucleic acid are both expressed by the exosome-producing cell.
Dependencies are inferred from the claim text.