Compositions and Methods for Identifying Secretory Antibody-Bound Microbes
AssigneeYale University
InventorsRichard Flavell, Noah Palm, Marcel de Zoete
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Abstract
The invention relates to the identification of secretory antibody-bound bacteria in the microbiota in a subject that influence the development and progression of inflammatory diseases and disorders. Thus, the invention relates to compositions and methods for detecting and identifying the constituents of a subject's microbiota, methods of modifying the constituents of the microbiota, and methods for treating inflammatory diseases and disorders in a subject in need thereof.
Claims33
A method of identifying a type of bacteria in the microbiota of a subject that contributes to the development or progression of an inflammatory disease or disorder in the subject, the method comprising the steps of:
- a. isolating a secretory antibody-bound bacteria from the subject's biological sample,
- b. amplifying bacterial nucleic acid from the secretory antibody-bound bacteria so isolated,
- c. determining the sequences of the amplicons so amplified,
- d. identifying the type of antibody-bound bacteria present in the subject's biological sample by identifying nucleic acid sequences that are indicative of particular types of bacteria.
A method of diagnosing an inflammatory disease or disorder in a subject in need thereof by identifying a type of bacteria in the microbiota of the subject that contributes to the development or progression of an inflammatory disease or disorder, the method comprising the steps of:
- a. isolating secretory an antibody-bound bacteria from the subject's biological sample,
- b. amplifying bacterial nucleic acid from the secretory antibody-bound bacteria so isolated,
- c. determining the sequences of the amplicons so amplified,
- d. identifying the type of antibody-bound bacteria present in the subject's biological sample by identifying nucleic acid sequences that are indicative of particular types of bacteria.
- wherein when the type of antibody-bound bacteria present in the subject's biological sample is a type of bacteria that contributes to the development or progression of an inflammatory disease or disorder, the subject is diagnosed with the inflammatory disease or disorder.
A method of treating an inflammatory disease or disorder associated with a secretory antibody-bound bacteria in the microbiota of a subject in need thereof, the method comprising administering to the subject at least one therapy to diminish the number of at least one type of bacteria that is over-represented in the microbiota of the subject.
Dependencies are inferred from the claim text.