Method for determining microbial susceptibility to antibiotic agents

A method for analyzing susceptibility of a glucose metabolizing microorganism to at least one antibiotic agent, comprising:

• a) cultivating said glucose metabolizing microorganism in a reference culture medium, comprising glucose and not comprising the antibiotic agent;
• and measuring the glucose concentration in the reference culture medium to obtain time-resolved glucose concentration data, wherein said cultivation extends for a period of time sufficient to observe a decrease in glucose concentration in the reference culture medium;
• b) simultaneously cultivating said glucose metabolizing microorganism in a culture medium contained in a culture medium container, in the presence of a predetermined concentration of an antibiotic agent and in the presence of glucose;
• c) measuring the concentration of glucose in the culture medium in said culture medium container at least when the glucose concentration in the reference culture medium has decreased below a predetermined threshold;
• and
• d) calculating a susceptibility score reflecting the susceptibility of the glucose metabolizing microorganism to the antibiotic agent based on a relation between the predetermined concentration of the agent with antibiotic activity, the glucose concentration data obtained in step c), and the time-resolved glucose concentration data obtained in step a).