Method for screening the activity of expressed polypeptides

Abstract

The present invention relates to a method for screening the activity of a polypeptide, and, equally, for producing a polypeptide with a desired activity by said screening, in an efficient and cost effective manner and with high sensitivity. The methods of the present invention comprise (i) expressing a polypeptide by cell-free in vitro translation and (ii) determining the activity of the expressed polypeptide by solution titration with an interaction member. The screening of a wild-type polypeptide and a mutant library derived there from can be used for identifying a activity of these polypeptides to an interaction member as a consequence of one or more point mutations. Furthermore, the present invention relates to methods for identifying polypeptides with therapeutically relevant properties as a consequence of the identified desired activity of a polypeptide from a mutant library.

Dependencies are inferred from the claim text.