Enantionselective enzymatic sulfoxidation of chiral arylsulfides

A product comprising the oxygenase subunit (StyA) that codes for a polypeptide with at least 80% sequence identity to SEQ ID NO 5 together with the reductase subunit (StyB) that codes for a polypeptide with at least 80% sequence identity to SEQ ID NO 6 or

• the oxygenase subunit (StyA) that codes for a polypeptide with at least 80% sequence identity to SEQ ID NO 5 together with a reductase subunit (StyB) that codes for the polypeptide of SEQ ID NO 6 or
• a oxygenase subunit (StyA) that codes for the polypeptide of SEQ ID NO 5 together with the reductase subunit (StyB) that codes for a polypeptide with at least 80% sequence identity to SEQ ID NO 6 or
• the oxygenase subunit (StyA) that codes for the polypeptide of SEQ ID NO 5 together with the reductase subunit (StyB) that codes for the polypeptide of SEQ ID NO 6
• for the enantioselective oxidation of a compound according to formula I
• wherein X is C or N, and
• wherein if X is N, R1 is absent
• and if X is C, R1 is selected form the group consisting of a H, NO2, a halogen, NH2, an C1 to C6 alkyl, or an an C1 to C6 O-alkyl, and
• wherein n is 0 or 1, and
• wherein R2 is an C1 to C6 alkyl and
• wherein R3 is a halogen or H
• into the S-sulfynil-enantiomer.

A method for enantioselective oxidation of a compound according to formula I

• into a S-sulfynil-enantiomer comprising
• providing a compound according to formula I,
• providing the oxygenase subunit (StyA) that codes for a polypeptide with at least 80% sequence identity to SEQ ID NO 5 together with the reductase subunit (StyB) that codes for a polypeptide with at least 80% sequence identity to SEQ ID NO 6 as polypeptide
• reacting said compound according to formula I with said polypeptide for 2-48 hours, with a pH in a range of 5-9 and the temperature is in a range of 15-40° C.