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US 2020/0370077 A1Published application
Random interesterification lipase
AssigneeAmano Enzyme Inc.
InventorsHiroki Fujioka, Saki Norifune, Tatsuya Hamada, Tetsuya Takahashi, Yusuke Tanaka, Toru Katase
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Abstract
An object of the present invention is to provide an enzyme showing random transesterification ability, which is suitable for food applications. Provided is a random transesterification lipase having high heat resistance in oils and fats.
Claims16
A lipase having the following enzymological properties:
- (1) action:
- catalyzing a random transesterification reaction;
- (2) molecular weight:
- having a molecular weight without N-glycan of about 36 kDa (by SDS-PAGE);
- (3) temperature stability in oils and fats:
- being stable at 60° C. or lower (by evaluation using tricaprylin and methyl stearate as substrates and also using an amount of methyl caprylate produced as an index);
- and
- (4) reactivity in oils and fats:
- showing the highest reactivity at 80° C. when allowed to react at 40 to 80° C. (by evaluation using cacao butter as a substrate and also using an amount of tripalmitin produced as an index).
6 more elements
A lipase having an amino acid sequence shown in SEQ ID NO:
- 2 or an amino acid sequence equivalent to the amino acid sequence.
A method for manufacturing a lipase for random transesterification, comprising the following steps (1) and (2):
- (1) culturing Candida pararugosa;
- and
- (2) collecting a lipase from a culture solution and/or cell bodies after the culture.
A lipase gene comprising any DNA selected from the group consisting of the following (a) to (c):
- (a) a DNA encoding an amino acid sequence of SEQ ID NO:
- 2 or an amino acid sequence equivalent to the amino acid sequence;
- (b) a DNA consisting of a base sequence of SEQ ID NO:
- 4 or 5;
- and
- (c) a DNA having a base sequence equivalent to the base sequence of SEQ ID NO:
- 4 or 5 and also encoding a protein having random transesterification activity.
4 more elements
Dependencies are inferred from the claim text.