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US 2020/0370035 A1Published application
Methods for in vitro site-directed mutagenesis using gene editing technologies
AssigneesChristiana Care Health Services, Inc., Novellusdx Ltd.
InventorsEric B. Kmiec, Michael Vidne, Gabi Tarcic
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Abstract
The invention relates to methods for performing in vitro site-directed mutagenesis of a targeted gene or genes. In another aspect, the invention includes in vitro site-directed mutagenesis kits comprising a ribonucleotide particle (RNP), an oligonucleotide, a buffer, a cell-free extract, and instructional material for use thereof.
Claims23
1-38. (canceled)
A method of performing mutagenesis of a target sequence, the method comprising:
- administering a mixture comprising:
- (a) a CRISPR delivery system comprising a crRNA and a Cas endonuclease;
- and
- (b) an oligonucleotide;
- to a cell thereby mutagenizing the target sequence.
2 more elements
A method of performing mutagenesis of a target sequence, the method comprising:
- (a) administering a CRISPR delivery system comprising a crRNA and a Cas endonuclease to a cell comprising the target sequence;
- and
- (b) administering before, during, or after the administering step (a) an oligonucleotide;
- thereby mutagenizing the target sequence.
1 more element
Dependencies are inferred from the claim text.