Apparatus And Method For Rapid Identification Of Microorganisms

A micro fluidic sampling device for rapid identification of a microorganism from a sample, comprising:

• a sample body having a main reservoir for receiving the sample and isolating the microorganism containing portion of said sample;
• a collection reservoir for receiving the microorganism containing portion of the sample from said sample reservoir;
• a plurality of reaction chambers each having a different reactive agent;
• and
• a capillary micro channel transferring the microorganism containing portion to each of said plurality of reaction chambers for reacting with the microorganism to indicate the presence of a microorganism in said reaction chamber.

A method for rapid identification of a microorganism in a sample, comprising the steps of;

• introducing a sample into a sample reservoir;
• isolating the microorganism containing portion of the sample;
• collecting the microorganism containing portion of the sample into a collection reservoir;
• introducing a different reactive agent in each of a plurality of reaction chambers;
• transferring the microorganism containing portion into each of the plurality of reaction chambers;
• and
• sequentially illuminating each of the plurality of reaction chambers for detecting the presence of a microorganism in the reaction chamber.

The method for rapid identification of a blood sample, comprising the steps of:

• injecting a blood sample into a main reservoir in a transparent cartridge or disk;
• separating the blood plasma liquid and pathogens from red and white blood cells;
• heating the separated the plasma liquid and pathogens with a laser to initiate a lysing process to crack the wall of the pathogens to release the DNA and RNAs;
• transferring the liquid containing the DMA and RNAs to a collection reservoir to reacting with an enzyme to cut the DNA and RNAs and to pick specific segment of the DNAs or RNAs;
• transferring the cut the DNA and RNAs and specific segment of the DNA or RNAs to a reaction chamber to react with CAS 12/13 protein;
• and
• illuminating the reaction chamber to defect the presence of pathogens.