Process for the purification of l-fucose from a fermentation broth
AssigneeJennewein Biotechnologie GmbH
InventorsMarkus HELFRICH, Stefan JENNEWEIN
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Abstract
The present invention concerns an efficient way to isolate L-fucose from a fermentation broth. The L-fucose contained in the fermentation broth is produced by microbial fermentation (bacterial or yeasts). The inventive process comprises a step of removing biomass from the fermentation broth, a step of subjecting the resulting solution to at least one of a cationic ion exchanger treatment and an anionic ion exchanger treatment and a step of removing salts after the ion exchanger treatment. The process can provide L-fucose in powder form, in granulated form as well as in form of L-fucose crystals.
Claims33
A process for the purification of L-fucose from a fermentation broth, comprising:
- removing biomass from a fermentation broth comprising L-fucose, wherein a clarified solution is provided,
- providing a purified solution by subjecting the clarified solution to
- a cationic ion exchanger treatment and
- an anionic ion exchanger treatment;
- and
- removing salt from the purified solution by electrodialysis and/or nanofiltration.
A composition comprising
- 1. at least 95.00 wt.-% of L-fucose;
- 2. at most 1.00 wt.-% organic solvent;
- and
- 3. at most 1.00 wt.-% salt.
A food composition, optionally an infant food formula, a toddler food formula or a medical nutrition product, comprising L-fucose and at least one other carbohydrate selected from galactooligosaccharides (GOS), fructooliogsoaccharides (FOS), inulin, lactulose, isomaltose, maltodextrin, lactose, and human milk oligosaccharides.
A liquid, ready-to-use infant or toddler nutrition product comprising L-fucose at a concentration of 1 mg/l to 2 g/l, a sialic acid and
- i) at least one neutral human milk oligosaccharide selected form the group consisting of 2′-fucosyllactose, 3-fucosyllactose, difucosyllactose, lacto-N-tetraose and lacto-N-neotetraose;
- and/or
- ii) at least one acidic human milk oligosaccharide selected from the group consisting of 3′-sialylactose or 6′-sialyllactose.
A spray-dried infant formula product comprising L-fucose at a concentration of 5 mg/kg to 15 g/kg, a sialic acid and
- i) at least one neutral human milk oligosaccharides selected form the group consisting of 2′-fucosyllactose, 3-fucosyllactose, difucosyllactose, lacto-N-tetraose and lacto-N-neotetraose;
- and/or
- ii) at least one acidic human milk oligosaccharide selected from the group consisting of 3′-sialyllactose and 6′-sialyllactose.
A dietary supplement comprising L-fucose and at least one neutral HMO selected from the group consisting of 2′-fucosyllactose, 3-fucosyllactose, difucosyllactose, lacto-N-triose II, lacto-N-tetraose and lacto-N-neotetraose, lacto-N-fucopentaose I.
A premix, optionally in the form of a spray-dried, granulated or liquid product, comprising a L-fucose and at least one substance selected from the group consisting of a protein source, a vitamin, an oil, a mineral, an enzyme, a further carbohydrate and a probiotic strain, wherein optionally the
- i) protein source is selected from the group consisting of whey, corn soya blend, protein hydrolysates and combinations thereof;
- and/or
- ii) vitamin is selected from the group consisting of vitamin A, thiamine, riboflavin, vitamin B12, folate and combinations thereof;
- and/or
- iii) oil is selected from the group consisting of palm oil, DHA, arachidonic acid and combinations thereof;
- and/or
- iv) mineral is selected from the group consisting of potassium chloride, potassium iodate, zinc oxide and combinations thereof;
- and/or
- v) enzyme is selected from the group consisting of amylase, amyloglucosidase and combinations thereof;
- and/or
- vi) carbohydrate is selected from the group consisting of a human milk oligosaccharide (HMO), a galactooligosaccharide (GOS), inulin (FOS), lactose, isomaltose, sialic acid, and combinations thereof;
- and/or
- vii) the probiotic strain is selected from the group consisting of (encapsulated) Lactobacillus, Bifidobacterium, Bacillus, yeast and combinations thereof.
Dependencies are inferred from the claim text.