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AssigneeNew York University
InventorBhubaneswar Mishra
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An exemplary system, method and computer-accessible medium for multiplexing base-calling of a plurality of nucleic acid molecules in the same flow cell is provided. When multiplexing for just two nucleic acid molecules, it can operate by selecting a first base call for a first nucleic acid molecule and a second base call for a second nucleic acid molecule, after having placed them in the same flow cell and obtaining the combined raw intensity output. It can use as prior the appropriate reference genome sequences from which the nucleic acid molecules can be derived in order to create a score function, which can additionally be constrained by various penalty functions. It can derive accuracy and speed by using a branch and bound strategy as well as by performing alignment and base-calling in one step per cycle.
A non-transitory computer-accessible medium having stored thereon computer-executable instructions for selecting a first base call for a first nucleic acid molecule and a second base call for a second nucleic acid molecule, wherein, when a computer arrangement executes the instructions, the computer arrangement is configured to perform procedures comprising:
A system for selecting a first base call for a first nucleic acid molecule and a second base call for a second nucleic acid molecule, comprising:
A method for selecting a first base call for a first nucleic acid molecule and a second base call for a second nucleic acid molecule, comprising:
Dependencies are inferred from the claim text.