Method for determining analyte-ligand binding on a sensor surface

A computer program comprising program code means for operating a sensor device to detect an interaction between an analyte and a ligand on a sensor surface according to the following method when the program is run on a computer, the method comprising:

• (a) providing a sensor surface having a ligand immobilized thereto wherein the ligand has a ligand density on the sensor surface configured to allow binding of analyte in the plurality of samples;
• (b) flowing a first sample solution over the sensor surface;
• and
• (c) detecting the presence or absence of binding of a first analyte to the ligand on the sensor surface;
• repeating steps (b) and (c) by flowing a subsequent sample solution that is the same or different than the first sample solution over the sensor surface to detect the presence or absence of binding between the ligand immobilized on the surface and a further analyte, the further analyte being the same or different than the first analyte, wherein the time between initiating the flowing of the first sample solution and flowing the second sample solution is less than forty seconds, and the contact time between the first sample solution and the immobilized ligand is less than 15 seconds wherein the contact time between the sample solution and the sensor surface having the ligand density is sufficient to allow binding between analyte in the plurality of samples and the ligand.