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US 2020/0347466 A1Published application
Methods and compositions for detection of enterovirus d68
AssigneeWashington University
InventorsGregory A. Storch, Todd N. Wylie, Kristine M. Wylie
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Abstract
The present disclosure is directed to improved methods and compositions for the detection of enterovirus D68.
Claims14
A method for detection of enterovirus D68 in a sample, the method comprising:
- a) contacting a nucleic acid obtained from the sample with an oligonucleotide primer comprising the sequence 5′-CACYGAACCAGARGAAGCCA-3′ (SEQ ID NO:3) and an oligonucleotide primer comprising the sequence 3′-AARGAATCATCCCGTCGAAATC-5′ (SEQ ID NO:4);
- b) exposing the contacted sample to a DNA amplification process that provides for production of a 98 nucleotide amplification product of the enterovirus D68 VP1 gene;
- and
- c) detecting the 98 nucleotide amplification product, wherein the presence of said amplification product indicates that the sample contained enterovirus D68.
1 more element
A method for detection of enterovirus D68 in a sample, the method comprising:
- a) contacting a nucleic acid obtained from the sample with an oligonucleotide primer pair capable of annealing to a sequence contained with to residues 2475 to 2572 of the enterovirus D68 sequence of SEQ ID NO:1 and providing a DNA amplification product therefrom of at least about 50 nucleotides to 98 nucleotides in length;
- b) exposing the contacted sample to a DNA amplification process that provides for production of a nucleotide amplification product of the enterovirus D68 VP1 gene;
- and
- c) detecting the amplification product, wherein the presence of said amplification product indicates that the sample contained enterovirus D68.
1 more element
Dependencies are inferred from the claim text.