Method for evaluating state of undifferentiated cell and utilization thereof
AssigneeSysmex Corporation
InventorsYuma OKA, Yuki AIHARA
Life of this patent
Estimating the term…
Abstract
A problem to be addressed is to provide a method that can evaluate the state of undifferentiated cells without destroying cells. The above problem is solved by providing a method for evaluating the state of undifferentiated cells. The method includes: measuring at least one miRNA selected from miR371, miR372, and miR373 in a liquid phase fraction of a liquid that contains undifferentiated cells; and evaluating a state of the undifferentiated cells on the basis of a measurement value of the miRNA.
Claims20
A method for evaluating a state of undifferentiated cells, the method comprising:
- measuring at least one miRNA selected from miR371, miR372, and miR373 in a liquid phase fraction of a liquid comprising undifferentiated cells;
- and
- evaluating a state of the undifferentiated cells on the basis of a measurement value of the miRNA.
A method for evaluating a state of undifferentiated cells, the method comprising:
- obtaining a liquid phase fraction of a liquid comprising undifferentiated cells, before an induction operation from primed state undifferentiated cells into naive state undifferentiated cells, and obtaining a first measurement value of at least one miRNA selected from miR371, miR372, and miR373 in the liquid phase fraction;
- obtaining a liquid phase fraction of the liquid comprising undifferentiated cells, during the induction operation from primed state undifferentiated cells into naive state undifferentiated cells, and obtaining a second measurement value of at least one miRNA selected from miR371, miR372, and miR373 in the liquid phase fraction;
- and
- comparing the first measurement value and the second measurement value with each other, thereby monitoring the induction operation of the undifferentiated cells.
A method for evaluating a state of undifferentiated cells, the method comprising:
- obtaining a liquid phase fraction of a liquid comprising undifferentiated cells, at a first time point during an induction operation from primed state undifferentiated cells into naive state undifferentiated cells, and obtaining a first measurement value of at least one miRNA selected from miR371, miR372, and miR373 in the liquid phase fraction;
- obtaining a liquid phase fraction of the liquid comprising undifferentiated cells, at a second time point during the induction operation from primed state undifferentiated cells into naive state undifferentiated cells, and obtaining a second measurement value of at least one miRNA selected from miR371, miR372, and miR373 in the liquid phase fraction;
- and
- comparing the first measurement value and the second measurement value with each other, thereby monitoring the induction operation of the undifferentiated cells.
Dependencies are inferred from the claim text.