Nucleic acid tests with temporal fluorescent signals

A device comprising:

• a sensor to detect a fluorescent signal from a reaction volume;
• and
• a processor to monitor the fluorescent signal during a nucleic acid amplification reaction in the reaction volume, the nucleic acid amplification reaction initiated with a nucleic acid sample and a fluorescent indicator to generate the fluorescent signal from an amplification product of the nucleic acid amplification reaction;
• the processor to indicate a plurality of increases in the fluorescent signal with respect to time during the nucleic acid amplification reaction, the plurality of increases associated with a plurality of nucleic acid sequences present in the nucleic acid sample at different concentrations.

A system comprising:

• a substrate defining a reaction volume;
• a sensor to detect a fluorescent signal from the reaction volume;
• and
• a processor to monitor the fluorescent signal during a nucleic acid amplification reaction in the reaction volume, the nucleic acid amplification reaction initiated with a nucleic acid sample and a fluorescent indicator to generate the fluorescent signal from an amplification product of the nucleic acid amplification reaction;
• the processor to indicate a plurality of increases in the fluorescent signal with respect to time during the nucleic acid amplification reaction, the processor to indicate an increase of the plurality of increases as an internal positive control of the nucleic acid sample.